KMID : 0880220130510050700
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Journal of Microbiology 2013 Volume.51 No. 5 p.700 ~ p.703
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Anaerobic Cellulolytic Rumen Fungal Populations in Goats Fed with and without Leucaena leucocephala Hybrid, as Determined by Real-Time PCR
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Ching Mun Kok
Chin Chin Sieo Hui Yin Tan Wan Zuhainis Saad Juan Boo Liang Yin Wan Ho
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Abstract
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The effect of Leucaena leucocephala hybrid-Bahru (LLB), which contains a high concentration of condensed tannins, on cellulolytic rumen fungal population in goats was investigated using real-time PCR. The fungal population in goats fed LLB was inhibited during the first 10 days of feeding, but after 15 days of feeding, there was a tremendous increase of fungal population (157.0 ¥ìg/ml), which was about fourfold more than that in control goats (39.7 ¥ìg/ml). However, after this period, the fungal population decreased continuously, and at 30 days of feeding, the fungal population (50.6 ¥ìg/ml) was not significantly different from that in control goats (55.4 ¥ìg/ml).
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KEYWORD
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Leucaena, condensed tannins, rumen fungal population, real-time PCR
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